%0 Figure %A Suñé-Auñón, Alejandro %A Jorge-Peñas, Alvaro %A Aguilar-Cuenca, Rocío %A Vicente-Manzanares, Miguel %A Van Oosterwyck, Hans %A Muñoz-Barrutia, Arrate %D 2017 %T Additional file 3: of Full L1-regularized Traction Force Microscopy over whole cells %U https://springernature.figshare.com/articles/figure/Additional_file_3_of_Full_L1-regularized_Traction_Force_Microscopy_over_whole_cells/5302369 %R 10.6084/m9.figshare.c.3850753_D3.v1 %2 https://springernature.figshare.com/ndownloader/files/9080176 %K Traction Force Microscopy %K Spatial domain %K Regularization %K Spatial resolution %X Ten samples frames illustrating the whole TFM experiment and comparing the traction recovery with the different regularization methods. For each frame: (Top row, left) CHO cell expressing Lifeact-GFP; (Top row, center) Pseudo-color image showing the fluorescent beads at the hydrogel surface. The beads of the unstressed and stressed hydrogels have been superposed and pseudo-colored in red and green, respectively; therefore, beads are colored in yellow when not displaced. The contrast of the pseudo-color images has been modified to highlight the areas with bead displacements; (Top row, right) Magnitude (in μm) and direction (arrows) of in-plane displacements estimated from the bead images. (Bottom row) Recovered traction magnitude (in Pa) and direction (arrows) using: (Left) Tikhonov regularization; (Center) L1-norm regularization; (Right) full L1-norm regularization. The outline of the mask used for traction recovery is shown in red (Top row, right) and white (for the rest). The scale bar represents 30 μm. Frame #5 corresponds to Fig. 7 in the main manuscript. (GIF 10461 kb) %I figshare