%0 Figure %A Haile, Simon %A Corbett, Richard %A MacLeod, Tina %A Bilobram, Steve %A Smailus, Duane %A Tsao, Philip %A Kirk, Heather %A McDonald, Helen %A Pandoh, Pawan %A Bala, Miruna %A Hirst, Martin %A Miller, Diane %A Moore, Richard %A Mungall, Andrew %A Schein, Jacquie %A Coope, Robin %A Ma, Yussanne %A Zhao, Yongjun %A Holt, Rob %A Jones, Steven %A Marra, Marco %D 2017 %T Additional file 17: Figure S13. of Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing %U https://springernature.figshare.com/articles/figure/Additional_file_17_Figure_S13_of_Increasing_quality_throughput_and_speed_of_sample_preparation_for_strand-specific_messenger_RNA_sequencing/5177776 %R 10.6084/m9.figshare.c.3819217_D8.v1 %2 https://springernature.figshare.com/ndownloader/files/8825113 %K Ampure XP magnetic beads %K Next-generation sequencing %K Library construction %K Strand-specific %K dUTP %K Uracil DNA N-Glycosylase %K Ligation %K mRNA %K RNA-seq %K Illumina %K Reverse transcriptase %X High correlation of expression between lower input and higher input libraries. Upper panel is for UHR data. Lower panel: High correlation of observed versus expected levels of ERCC spike-in synthetic RNAs. Pearson’s correlation coefficient was calculated pair-wise among the libraries (UHR data) and between what was measured in the libraries versus theoretically expected levels for the ERCC RNAs. n = 2. (JPEG 50 kb) %I figshare