10.6084/m9.figshare.c.3807619_D1.v1
Anderson Webb
Anderson
Webb
Bradley Gao
Bradley
Gao
Zachary Goldsmith
Zachary
Goldsmith
Andrew Irvine
Andrew
Irvine
Nabil Saleh
Nabil
Saleh
Ryan Lee
Ryan
Lee
Justin Lendermon
Justin
Lendermon
Rajini Bheemreddy
Rajini
Bheemreddy
Qiuhua Zhang
Qiuhua
Zhang
Rachel Brennan
Rachel
Brennan
Dianna Johnson
Dianna
Johnson
Jena Steinle
Jena
Steinle
Matthew Wilson
Matthew
Wilson
Vanessa Morales-Tirado
Vanessa
Morales-Tirado
Additional file 1: Figure S1. of Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma
Springer Nature
2017
Matrix metalloproteinases
MMP-2
MMP-9
Retinoblastoma
Therapy
Metastasis
VEGF
TGF-β1
2017-06-20 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Inhibition_of_MMP-2_and_MMP-9_decreases_cellular_migration_and_angiogenesis_in_in_vitro_models_of_retinoblastoma/5132887
Inhibition of MMP-2 or MMP-9 reduced Rb viability and cell cycle progression. a, Y79 cells were cultured in the presence or absence of the MMPI overnight. Next day, we collected cells and assessed viability by Trypan Blue exclusion. Chemical inhibition of Y79 with MMPI significantly reduced cell yield when compared to untreated cells. b, RNA interference was used to confirm on-target effects of MMPIs. Y79 were cultured in the presence of either MMP2 or MMP9 siRNA. MMP2 and MMP9 knockdown groups showed significant reduction in cell yield, illustrating an on-target effect of MMPI. c, Imaging flow cytometry analysis showed inhibition of MMP9 prevents progression of Rb cell division using nuclear DRAQ5™ labeling. Bar graphs indicate results ± SEM to control. **p < 0.005. (TIF 434 kb)