10.6084/m9.figshare.c.3799402_D1.v1 Giulia Cesi Giulia Cesi Geoffroy Walbrecq Geoffroy Walbrecq Andreas Zimmer Andreas Zimmer Stephanie Kreis Stephanie Kreis Claude Haan Claude Haan Additional file 1: Figure S1. of ROS production induced by BRAF inhibitor treatment rewires metabolic processes affecting cell growth of melanoma cells Springer Nature 2017 Melanoma Metabolism RAS/RAF/MEK/ERK pathway NRAS BRAF inhibitors MEK inhibitors Pyruvate dehydrogenase Pyruvate dehydrogenase kinases Reactive oxygen species PDK inhibitors 2017-06-08 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_ROS_production_induced_by_BRAF_inhibitor_treatment_rewires_metabolic_processes_affecting_cell_growth_of_melanoma_cells/5097055 PLX4032 and GSK2118436 do not induce up-regulation of HIF-1α protein in BRAFV600E melanoma cells. Western blot analysis of A375, IGR37 and 501Mel cells (BRAFV600E) treated with 1 μM of PLX4032 and 100 nM of GSK2118432 for the indicated time points. HIF-1α protein was not detectable in all three cell lines. Positive control: A375 short term hypoxia. (PDF 155 kb)