Additional file 4: Figure S3. of Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
Samantha de Witte
Ana Merino
Marcella Franquesa
Tanja Strini
Johanna van Zoggel
Sander Korevaar
Franka Luk
Madhu Gargesha
Lisa O’Flynn
Debashish Roy
Steve Elliman
Philip Newsome
Carla Baan
Martin Hoogduijn
10.6084/m9.figshare.c.3799369_D4.v1
https://springernature.figshare.com/articles/figure/Additional_file_4_Figure_S3_of_Cytokine_treatment_optimises_the_immunotherapeutic_effects_of_umbilical_cord-derived_MSC_for_treatment_of_inflammatory_liver_disease/5096977
Ex vivo experimental scheme. UcMSC were pre-treated for 3 days. Medium was then refreshed and the cells were left overnight in the incubator. The following day, livers were collected from healthy C57BL/6 mice and directly after isolation cut into slices (diameter = 1 cm and thickness = 150 μm) and placed in the wells on top of the pre-treated ucMSC in the presence of LPS. The liver slices were left overnight in the incubator on a shaker (50 rpm). After 24 hours, supernatant and liver slices were harvested. (TIF 4600 kb)
2017-06-08 05:00:00
Immunogenic
Immunomodulatory
Mesenchymal stromal cell
Optimising