MOESM2 of Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells GeisFranziska GallaMelanie HoffmannDirk KuehleJohannes ZychlinskiDaniela MaetzigTobias SchottJuliane SchwarzerAdrian GoffinetChristine GoffStephen SchambachAxel 2017 Additional file 2. CSA exhibits no changes in cell growth or apoptosis at a concentration of 10 ¾M in iPSC. iPSC were treated with CSA at indicated concentrations and washed after 12 hours (n = 3). (A) Cell counts are shown after 48 hours. (B) Cells were stained for AnnexinV and PI after 48 hours and percentages of fluorescence positive cells are shown. Camptothecin served as a positive control for cytotoxicity.