Additional file 6: Figure S5. of Rp58 and p27kip1 coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex Olivier Clément Isabel Hemming Ivan Gladwyn-Ng Zhengdong Qu Shan Li Michael Piper Julian Heng 10.6084/m9.figshare.c.3780524_D6.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_Figure_S5_of_Rp58_and_p27kip1_coordinate_cell_cycle_exit_and_neuronal_migration_within_the_embryonic_mouse_cerebral_cortex/5009489 Forced expression of p27kip1 enhances radial migration and alters the morphological characteristics of embryonic cortical neurons. (A-B) Forced expression of p27kip1 leads to enhanced radial migration, observed as a significant increase in the proportion of cells arriving in the CP (F2,12 = 7.245, p = 0.0085; Two-way ANOVA followed by Bonferroni’s posthoc t-test; *p < 0.05) (C). (D) The intracortical positioning of p27kip1-overexpressing cells is also affected, observed as a significant increase in the proportion of cells within the upper CP (uCP) (F2,12 = 2.645, p = 0.118; Two-way ANOVA followed by Bonferroni’s posthoc t-test; *p < 0.05). (E-F) Representative images of IZ neurons within each treatment condition. (G) The distribution of cell shapes within the IZ are not significantly affected by p27kip1 overexpression (F2,24 = 1.8, p = 0.194; Two-way ANOVA. (H-J) Representative images of CP neurons within each treatment condition. Pie charts representing the lengths of the leading processes of uni/bipolar shaped neurons within the CP are represented for each treatment condition. (J) There is a significant interaction between the distribution of CP cell shapes and p27kip1 overexpression (F2,27 = 5.0, p = 0.0147; Two-way ANOVA), but the differences for a given cell shape was not significantly different upon posthoc t-testing. (K) The leading processes of p27kip1-overexpressing neurons were significantly shorter when compared with control (p < 0.0001 student’s t-test, two-tailed; n > 285 neurons per condition). Scale bars represent 100 μm (B) and 20 μm (F, I) respectively. (PDF 331 kb) 2017-05-15 05:00:00 Cerebral Cortex Transcription Factor Neurodevelopment Neurogenesis Radial migration Neuronal morphology