Additional file 6: Figure S6. of Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129 Wen-Bo Zeng Hai-Fei Jiang Ya-Dong Gang Yi-Ge Song Zhang-Zhou Shen Hong Yang Xiao Dong Yong-Lu Tian Rong-Jun Ni Yaping Liu Na Tang Xinyan Li Xuan Jiang Ding Gao Michelle Androulakis Xiao-Bin He Hui-Min Xia Ying-Zi Ming Youming Lu Jiang-Ning Zhou Chen Zhang Xue-Shan Xia Yousheng Shu Shao-Qun Zeng Fuqiang Xu Fei Zhao Min-Hua Luo 10.6084/m9.figshare.c.3778625_D7.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_Figure_S6_of_Anterograde_monosynaptic_transneuronal_tracers_derived_from_herpes_simplex_virus_1_strain_H129/5004641 Invasion and transmission of H129-G4 in the VPM-S1 circuit. (a) Simplified schema of the VPM-S1 circuit. VPM, ventral posteromedial thalamic nucleus; nRT, nucleus of reticular thalamus; S1, primary somatosensory cortex; IV, V and VI, layer 4, 5 and 6 of the cortex. (b) Representative tracing results of H129-G4 in VPM-S1 circuit. H129-G4 (1 × 106 pfu in 200 nl) was injected into the VPM of wild-type C57BL/6 mice together with Alexa Fluor 594-conjugated CTB (CTB, red). The animals were perfused at the indicated time points, and representative images of the coronal brain slice at the VPM-S1 regions are shown. The boxed areas are magnified and presented in the right panel. The layers of the S1 cortex were determined according to NeuN staining and indicated by the dotted lines. (PDF 503 kb) 2017-05-12 05:00:00 Herpes simplex virus type 1 (HSV-1) H129 strain Anterograde Neuronal tracer H129 ΔTK tdT Monosynaptic H129-G4 Multisynaptic