Additional file 3: Figure S3. of Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia RakotomamonjyJennifer BrunnerMolly JüschkeChristoph ZangKeling HuangEric ReichardtLouis ChennAnjen 2017 Loss of primary cilia and mislocalization of primary ciliogenesis initiator Rab11 following Afadin deletion. (A) Quantification of primary cilium number from electronic microscopy analysis at E12.5 confirms the loss of primary cilia specifically at the dorsal neuroepithelium (mean ± s.e.m. Unpaired t-test; n = 4 controls, 20 to 24 images per embryo; n = 3 mutants 15 to 24 images per embryo). (B) Immunofluorescence for the small GTPase Rab11 in control (upper panels) and mutant (lower panels) reveals that Afadin allows the proper distribution of this initiator of primary ciliogenesis. (TIF 1961 kb)