%0 Journal Article %A Mu, Juan %A Zhu, Dongmei %A Shen, Zhaoxia %A Ning, Shilong %A Liu, Yun %A Chen, Juan %A Li, Yuan %A Li, Zhong %D 2017 %T Additional file 4: Figure S2. of The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S2_of_The_repressive_effect_of_miR-148a_on_Wnt_-catenin_signaling_involved_in_Glabridin-induced_anti-angiogenesis_in_human_breast_cancer_cells/4963934 %R 10.6084/m9.figshare.c.3769727_D4.v1 %2 https://springernature.figshare.com/ndownloader/files/8349608 %K Breast cancer %K Angiogenesis %K Glabridin %K microRNA-148a %K Wnt/β-catenin signaling %X MDA-MB-231 cells were pretreated with 0, or 20 μM GLA for 48 h, then the media was removed, the cells were washed with 1× PBS, followed by replacement with fresh media with 1% FBS for 24 h. (A)The expression of miR-148a was analyzed by qRT-PCR (mean ± SD, n = 3). (B-C) Western blot analyses the relative protein levels of Wnt1, β-catenin, and non-phospho (active) β-catenin (mean ± SD, n = 3). (D) The ELISA was used to detect the secretion of VEGF (mean ± SD, n = 3); **P < 0.01 and ***P < 0.001 compared with the control media or cells. (DOCX 322 kb) %I figshare