10.6084/m9.figshare.c.3769727_D4.v1 Juan Mu Juan Mu Dongmei Zhu Dongmei Zhu Zhaoxia Shen Zhaoxia Shen Shilong Ning Shilong Ning Yun Liu Yun Liu Juan Chen Juan Chen Yuan Li Yuan Li Zhong Li Zhong Li Additional file 4: Figure S2. of The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells Springer Nature 2017 Breast cancer Angiogenesis Glabridin microRNA-148a Wnt/β-catenin signaling 2017-05-02 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S2_of_The_repressive_effect_of_miR-148a_on_Wnt_-catenin_signaling_involved_in_Glabridin-induced_anti-angiogenesis_in_human_breast_cancer_cells/4963934 MDA-MB-231 cells were pretreated with 0, or 20 μM GLA for 48 h, then the media was removed, the cells were washed with 1× PBS, followed by replacement with fresh media with 1% FBS for 24 h. (A)The expression of miR-148a was analyzed by qRT-PCR (mean ± SD, n = 3). (B-C) Western blot analyses the relative protein levels of Wnt1, β-catenin, and non-phospho (active) β-catenin (mean ± SD, n = 3). (D) The ELISA was used to detect the secretion of VEGF (mean ± SD, n = 3); **P < 0.01 and ***P < 0.001 compared with the control media or cells. (DOCX 322 kb)