10.6084/m9.figshare.c.3769727_D4.v1
Juan Mu
Juan
Mu
Dongmei Zhu
Dongmei
Zhu
Zhaoxia Shen
Zhaoxia
Shen
Shilong Ning
Shilong
Ning
Yun Liu
Yun
Liu
Juan Chen
Juan
Chen
Yuan Li
Yuan
Li
Zhong Li
Zhong
Li
Additional file 4: Figure S2. of The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells
Springer Nature
2017
Breast cancer
Angiogenesis
Glabridin
microRNA-148a
Wnt/β-catenin signaling
2017-05-02 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S2_of_The_repressive_effect_of_miR-148a_on_Wnt_-catenin_signaling_involved_in_Glabridin-induced_anti-angiogenesis_in_human_breast_cancer_cells/4963934
MDA-MB-231 cells were pretreated with 0, or 20 μM GLA for 48 h, then the media was removed, the cells were washed with 1× PBS, followed by replacement with fresh media with 1% FBS for 24 h. (A)The expression of miR-148a was analyzed by qRT-PCR (mean ± SD, n = 3). (B-C) Western blot analyses the relative protein levels of Wnt1, β-catenin, and non-phospho (active) β-catenin (mean ± SD, n = 3). (D) The ELISA was used to detect the secretion of VEGF (mean ± SD, n = 3); **P < 0.01 and ***P < 0.001 compared with the control media or cells. (DOCX 322 kb)