Llorens, Franc Thüne, Katrin Sikorska, Beata Schmitz, Matthias Tahir, Waqas Fernández-Borges, Natalia Cramm, Maria Gotzmann, Nadine Carmona, Margarita Streichenberger, Nathalie Michel, Uwe Zafar, Saima Schuetz, Anna-Lena Rajput, Ashish Andréoletti, Olivier Bonn, Stefan Fischer, Andre Liberski, Pawel Torres, Juan Ferrer, Isidre Zerr, Inga Additional file 5: Figure S4. of Altered Ca2+ homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease Alteration of autophagy related genes in sCJD. (A) Altered expression of genes involved in autophagy in the cortex of the tg340-PRNP129MM mice at 120 (pre-clinical) and 180 (clinical) days after inoculation with sCJD MM1 brain homogenates. (B) qPCR analysis of the autophagy activators hspa8 and hspb8 in the sCJD infected tg340-PRNP129MM mice at 180 dpi. (C) Western.-blot and densitometric analysis of the autophagy related proteins DJ-1, LC3, ATG5 and LAMP2 in the frontal cortex of control and sCJD MM1 and sCJD VV2 cases. (D) qPCR analysis of the autophagy-related genes HSPA8, HSPB8, PARK (DJ-1) and LAMP2 in the frontal cortex of control and sCJD MM1 and sCJDVV2 cases. Unpaired t-test (95% CI) was used for the comparisons of the two groups. ANOVA test followed by post-test Tukey’s Multiple Comparison Test was used to compare the values from different groups. P values for the comparisons of the three groups are indicated in the figure:*p < 0.05; **p < 0.01; ***p < 0.001. (PPTX 244 kb) Creutzfeldt-Jakob disease;Prion protein;Calpain;Cathepsin;Calcium;Ca2+ 2017-04-27
    https://springernature.figshare.com/articles/presentation/Additional_file_5_Figure_S4_of_Altered_Ca2_homeostasis_induces_Calpain-Cathepsin_axis_activation_in_sporadic_Creutzfeldt-Jakob_disease/4929173
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