10.6084/m9.figshare.c.3758096_D5.v1 Franc Llorens Franc Llorens Katrin Thüne Katrin Thüne Beata Sikorska Beata Sikorska Matthias Schmitz Matthias Schmitz Waqas Tahir Waqas Tahir Natalia Fernández-Borges Natalia Fernández-Borges Maria Cramm Maria Cramm Nadine Gotzmann Nadine Gotzmann Margarita Carmona Margarita Carmona Nathalie Streichenberger Nathalie Streichenberger Uwe Michel Uwe Michel Saima Zafar Saima Zafar Anna-Lena Schuetz Anna-Lena Schuetz Ashish Rajput Ashish Rajput Olivier Andréoletti Olivier Andréoletti Stefan Bonn Stefan Bonn Andre Fischer Andre Fischer Pawel Liberski Pawel Liberski Juan Torres Juan Torres Isidre Ferrer Isidre Ferrer Inga Zerr Inga Zerr Additional file 5: Figure S4. of Altered Ca2+ homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease Springer Nature 2017 Creutzfeldt-Jakob disease Prion protein Calpain Cathepsin Calcium Ca2+ 2017-04-27 05:00:00 Presentation https://springernature.figshare.com/articles/presentation/Additional_file_5_Figure_S4_of_Altered_Ca2_homeostasis_induces_Calpain-Cathepsin_axis_activation_in_sporadic_Creutzfeldt-Jakob_disease/4929173 Alteration of autophagy related genes in sCJD. (A) Altered expression of genes involved in autophagy in the cortex of the tg340-PRNP129MM mice at 120 (pre-clinical) and 180 (clinical) days after inoculation with sCJD MM1 brain homogenates. (B) qPCR analysis of the autophagy activators hspa8 and hspb8 in the sCJD infected tg340-PRNP129MM mice at 180 dpi. (C) Western.-blot and densitometric analysis of the autophagy related proteins DJ-1, LC3, ATG5 and LAMP2 in the frontal cortex of control and sCJD MM1 and sCJD VV2 cases. (D) qPCR analysis of the autophagy-related genes HSPA8, HSPB8, PARK (DJ-1) and LAMP2 in the frontal cortex of control and sCJD MM1 and sCJDVV2 cases. Unpaired t-test (95% CI) was used for the comparisons of the two groups. ANOVA test followed by post-test Tukey’s Multiple Comparison Test was used to compare the values from different groups. P values for the comparisons of the three groups are indicated in the figure:*p < 0.05; **p < 0.01; ***p < 0.001. (PPTX 244 kb)