%0 Journal Article %A Liu, Qiang %A Cui, Xiang %A Yu, Xi %A Bian, Bai-Shi-Jiao %A Qian, Feng %A Hu, Xu-gang %A Ji, Cheng-dong %A Yang, Lang %A Ren, Yong %A Cui, Wei %A Zhang, Xia %A Zhang, Peng %A Wang, Ji %A Cui, You-hong %A Bian, Xiu-wu %D 2017 %T Additional file 1: of Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Cripto-1_acts_as_a_functional_marker_of_cancer_stem-like_cells_and_predicts_prognosis_of_the_patients_in_esophageal_squamous_cell_carcinoma/4902419 %R 10.6084/m9.figshare.c.3749432_D1.v1 %2 https://springernature.figshare.com/ndownloader/files/8241779 %K Cripto-1 %K Esophageal squamous cell carcinoma %K Cancer stem-like cells %K Metastasis %K Prognosis %X Supplementary materials and methods, including Quantitative RT-PCR, Western blotting, Flow cytometry, Silencing CR-1 with shRNA, and Immunofluorescence staining. Table S1. Primer sequences for qRT-PCR assay. Table S2. shRNA sequences targeting CR-1. Table S3. Incidence of tumor formation in nude mice injected with ESCC cells. Table S4. The frequency of lung metastasis (1x104 cells/mouse). Table S5. Significant difference of CR-1 expression between carcinoma and adjacent normal tissues. Figure S1. The levels of CR-1expression and the silencing efficiency of CR-1 shRNA in ESCC cells. Figure S2. Silencing CR-1 expression significantly represses the self-renewal and tumorigenicity inTE-1 cells. Figure S3. Suppression of CR-1 expression inhibits the invasive and metastatic capabilities of TE-1 cells in vitro and in vivo. Figure S4. The effect of silencing CR-1 on the expression of MMPs in EC109 cells. (DOC 1602 kb) %I figshare