10.6084/m9.figshare.c.3740003_D9.v1
Han Rauwerda
Han
Rauwerda
Johanna Pagano
Johanna
Pagano
Wim de Leeuw
Wim
de Leeuw
Wim Ensink
Wim
Ensink
Ulrike Nehrdich
Ulrike
Nehrdich
Mark de Jong
Mark de
Jong
Martijs Jonker
Martijs
Jonker
Herman Spaink
Herman
Spaink
Timo Breit
Timo
Breit
Additional file 18: of Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos
Springer Nature
2017
Maternal RNA
Individual transcriptome
Zebrafish
Danio rerio
Embryogenesis
Gastrulation
2017-04-11 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_18_of_Transcriptome_dynamics_in_early_zebrafish_embryogenesis_determined_by_high-resolution_time_course_analysis_of_180_successive_individual_zebrafish_embryos/4867907
Example of the determination of a stopping point and a starting point. For an explanation see Methods, paragraph “Identification of starting and stopping points”. lf1 = linear fit on all samples; lof1 = lowest fit on all samples; grey lines indicate intersect of lf1 and lof1; L1: embryo with the largest difference between lf1 and lof1; for stopping genes lf2 = linear fit of L1 to 179; for starting genes lf2 = linear fit of 1 to L1; embryos marked with an asterisk: ‘not present’ probes in the last (stopping) 15 respectively in the first (starting) 15 embryos; horizontal black line: median probe intensity of embryos marked with an asterisk; dashed line: intersect of lf2 and the horizontal black line indicating the stopping respectively the starting point. (PDF 131 kb)