10.6084/m9.figshare.c.3740003_D9.v1 Han Rauwerda Han Rauwerda Johanna Pagano Johanna Pagano Wim de Leeuw Wim de Leeuw Wim Ensink Wim Ensink Ulrike Nehrdich Ulrike Nehrdich Mark de Jong Mark de Jong Martijs Jonker Martijs Jonker Herman Spaink Herman Spaink Timo Breit Timo Breit Additional file 18: of Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos Springer Nature 2017 Maternal RNA Individual transcriptome Zebrafish Danio rerio Embryogenesis Gastrulation 2017-04-11 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_18_of_Transcriptome_dynamics_in_early_zebrafish_embryogenesis_determined_by_high-resolution_time_course_analysis_of_180_successive_individual_zebrafish_embryos/4867907 Example of the determination of a stopping point and a starting point. For an explanation see Methods, paragraph “Identification of starting and stopping points”. lf1 = linear fit on all samples; lof1 = lowest fit on all samples; grey lines indicate intersect of lf1 and lof1; L1: embryo with the largest difference between lf1 and lof1; for stopping genes lf2 = linear fit of L1 to 179; for starting genes lf2 = linear fit of 1 to L1; embryos marked with an asterisk: ‘not present’ probes in the last (stopping) 15 respectively in the first (starting) 15 embryos; horizontal black line: median probe intensity of embryos marked with an asterisk; dashed line: intersect of lf2 and the horizontal black line indicating the stopping respectively the starting point. (PDF 131 kb)