Additional file 1: Figure S1. of Vaccination with poly(IC:LC) and peptide-pulsed autologous dendritic cells in patients with pancreatic cancer
Shikhar Mehrotra
Carolyn Britten
Steve Chin
Elizabeth Garrett-Mayer
Colleen Cloud
Mingli Li
Gina Scurti
Mohamed Salem
Michelle Nelson
Melanie Thomas
Chrystal Paulos
Andres Salazar
Michael Nishimura
Mark Rubinstein
Zihai Li
David Cole
10.6084/m9.figshare.c.3737779_D1.v1
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_Vaccination_with_poly_IC_LC_and_peptide-pulsed_autologous_dendritic_cells_in_patients_with_pancreatic_cancer/4832077
Cytokine secretion by DCs. Frozen DCs obtained from patients were thawed and cultured with GM-CSF/IL-4 overnight before adding poly (IC:LC) for maturation overnight. The supernatant was collected after 16Â hrs. and evaluated for cytokines IL12 (upper panel), and IL10 (lower panel) as per the manufacturerâs protocol. Figure S2. PFS and OS details. The raw data showing details of progression free survival (PFS) and the median overall survival (OS) is presented in tabular form. Figure S3. Gating scheme for flow cytometry analysis. PBMC was obtained from patients post vaccination and cryopreserved cells were stained using the multiple fluorochrome-conjugated antibodies. Cells were gated based on singlets (FSC-A vs FSC-H), size (SSC vs FSC-H), a live-dead stain (L/D), and subsequently markers to determine specific cell phenotypes. A) CD3+ T cells were phenotyped for CD4 and CD8. B) CD19 B cells were identified. C) NK cells were identified based on their CD56 and CD16 expression. The data was acquired using BD FACS Aria and analyzed using FlowJo software. (PDF 351Â kb)
2017-04-07 05:00:00
CD 19 B cells
cytokines IL 12
tabular form
cryopreserved cells
FSC-A
FSC-H
NK cells
pancreatic cancer Cytokine secretion
patients post vaccination
GM-CSF
CD 8. B
OS details
CD 4
Figure S 2. PFS
SSC
PBMC
cell phenotypes
live-dead stain
FlowJo software
CD 16 expression
flow cytometry analysis
CD 56
DC
peptide-pulsed autologous dendritic cells
16Â hrs
IL 10
PDF 351Â kb
BD FACS Aria
fluorochrome-conjugated antibodies
IC
Figure S 3. Gating scheme