Additional file 1: Figure S3. of Anti-tumor effects of everolimus and metformin are complementary and glucose-dependent in breast cancer cells AriaansGerke JalvingMathilde VriesEmma JongSteven 2017 Uncoupling of the mitochondrial respiration with FCCP after treatment with metformin and everolimus for 48 h. Using the seahorse XF analyzer, the basal OCR and maximal OCR after uncoupling of mitochondrial respiration with FCCP of MCF-7 (A) and MDA-MB-231 cells (B) in response to 48 h of metformin or everolimus treatment was determined. Basal OCR in untreated cells was set at 100% as a reference to which all other mitochondrial respiration values of the same glucose concentration group were correlated. Data are presented as mean ± SD of three different experiments. (PDF 69 kb)