10.6084/m9.figshare.c.3723067_D2.v1 Michal Arbel-Ornath Michal Arbel-Ornath Eloise Hudry Eloise Hudry Josiah Boivin Josiah Boivin Tadafumi Hashimoto Tadafumi Hashimoto Shuko Takeda Shuko Takeda Kishore Kuchibhotla Kishore Kuchibhotla Steven Hou Steven Hou Carli Lattarulo Carli Lattarulo Arianna Belcher Arianna Belcher Naomi Shakerdge Naomi Shakerdge Pariss Trujillo Pariss Trujillo Alona Muzikansky Alona Muzikansky Rebecca Betensky Rebecca Betensky Bradley Hyman Bradley Hyman Brian Bacskai Brian Bacskai Additional file 2: Figure S2. of Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain Springer Nature 2017 Alzheimer’s disease Amyloid β oligomers Calcium In vivo imaging 2017-03-21 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_Soluble_oligomeric_amyloid-_induces_calcium_dyshomeostasis_that_precedes_synapse_loss_in_the_living_mouse_brain/4776169 Time course of oAβ-induced increase in resting calcium levels after acute exposure with TgCM on a healthy brain. C57BL/6 mice were injected with AAV-CBA-YC3.6 into the somatosensory cortex and acutely exposed to TgCM containing oAβ. The changes in YFP/CFP ratios in the neurites were measured at baseline before treatment and 30 min, 1 h and 5 h after topical application of TgCM. A progressive increase in the levels of resting calcium (YFP/CFP ratio and the actual calcium levels on the lower and upper x-axis, respectively) was detected that reached a peak after 1 h (20.6% of overloaded neurites), before returning to baseline levels after 5 h (1.7% overloaded neurites). (TIF 37844 kb)