Additional file 2: Table S1. of Analysis of monocyte infiltration in MPTP mice reveals that microglial CX3CR1 protects against neurotoxic over-induction of monocyte-attracting CCL2 by astrocytes ParillaudVincent LornetGuillaume MonnetYann PrivatAnne-Laure HaddadAndrei BrochardVanessa BekaertAmaury de ChanvilleCamille HirschEtienne CombadièreChristophe HunotStéphane LobsigerChristian 2017 RNA profiling of chemokines in the laser-microdissected substantia nigra of the MPTP mouse model. Shown are results from the RT-qPCR profiling of 96 genes using TaqMan 384-well microfluidic cards, to assess all 61 chemokine family members (37 ligands, 24 receptors) as well as 32 other selected neuroinflammation-linked genes, normalized to Hprt1. RNA profiling was done in laser-microdissected SNpc at 2, 4, and 7 days after acute MPTP intoxication in adult 12 weeks old C57BL/6J males and compared to saline-injected controls (n = 9 mice per condition; as biological replicates). qPCR results are shown as normalized and averaged fold-changes (FC) to saline controls. In addition, the raw averaged Ct values of both the control and the 2 days (MPTP) samples are shown. Ct values of >30 are called absent (Abs). Only FC values that are statistically significant (P < 0.05; qBasePlus, Biogazelle) are indicated. Of note, the large FC values can be explained by both the enrichment provided by the LMD and the often very low expression in baseline control conditions. (nc, expressed but no significant change and N.D., non-determined) (* asterisks mark unclear corresponding receptors; Griffith et al. 2014). (PDF 163 kb)