MOESM2 of False-negative malaria rapid diagnostic tests in Rwanda: impact of Plasmodium falciparum isolates lacking hrp2 and declining malaria transmission KozyckiChristina UmulisaNoella RulisaStephen MwikaragoEmil MusabyimanaJean HabimanaJean KaremaCorine KrogstadDonald 2017 Additional file 2. PCR products visualized on an agarose gel. DNA is from two thick-smear positive subjects with negative HRP2-based RDTs. Lane 1: 100 bp marker. Lane 2: PCR targeting hrp2 with DNA from subject #1 (shows amplicon of expected size of ~900 bp). Lanes 3–5: PCR with DNA from subject #2. Lane 3 shows the absence of hrp2 amplicons. Lane 4 shows results of multiplex PCR for 18S rRNA with an amplicon of 276 bp (expected size for P. falciparum). Lane 5 shows results of nested PCR with species-specific primers for P. falciparum 18S rRNA with an amplicon of the expected size of 205 bp.