%0 Journal Article %A Kozycki, Christina %A Umulisa, Noella %A Rulisa, Stephen %A Mwikarago, Emil %A Musabyimana, Jean %A Habimana, Jean %A Karema, Corine %A Krogstad, Donald %D 2017 %T MOESM2 of False-negative malaria rapid diagnostic tests in Rwanda: impact of Plasmodium falciparum isolates lacking hrp2 and declining malaria transmission %U https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_False-negative_malaria_rapid_diagnostic_tests_in_Rwanda_impact_of_Plasmodium_falciparum_isolates_lacking_hrp2_and_declining_malaria_transmission/4768789 %R 10.6084/m9.figshare.c.3721600_D2.v1 %2 https://springernature.figshare.com/ndownloader/files/7830943 %K Malaria %K Plasmodium falciparum %K Rapid diagnostic test (RDT) %K Histidine rich protein 2 (HRP2) %K Plasmodium lactate dehydrogenase (pLDH) %K Sensitivity %K Polymerase chain reaction (PCR) %K Rwanda %X Additional file 2. PCR products visualized on an agarose gel. DNA is from two thick-smear positive subjects with negative HRP2-based RDTs. Lane 1: 100 bp marker. Lane 2: PCR targeting hrp2 with DNA from subject #1 (shows amplicon of expected size of ~900 bp). Lanes 3–5: PCR with DNA from subject #2. Lane 3 shows the absence of hrp2 amplicons. Lane 4 shows results of multiplex PCR for 18S rRNA with an amplicon of 276 bp (expected size for P. falciparum). Lane 5 shows results of nested PCR with species-specific primers for P. falciparum 18S rRNA with an amplicon of the expected size of 205 bp. %I figshare