10.6084/m9.figshare.c.3709102_D7.v1 Yanqing Liu Yanqing Liu Rui Liu Rui Liu Fei Yang Fei Yang Rongjie Cheng Rongjie Cheng Xiaorui Chen Xiaorui Chen Shufang Cui Shufang Cui Yuanyuan Gu Yuanyuan Gu Wu Sun Wu Sun Chaoying You Chaoying You Zhijian Liu Zhijian Liu Feng Sun Feng Sun Yanbo Wang Yanbo Wang Zheng Fu Zheng Fu Chao Ye Chao Ye Chenyu Zhang Chenyu Zhang Jing Li Jing Li Xi Chen Xi Chen Additional file 6: Figure S3. of miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1 Springer Nature 2017 Colorectal cancer microRNA miR-19a TIA1 2017-03-04 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_6_Figure_S3_of_miR-19a_promotes_colorectal_cancer_proliferation_and_migration_by_targeting_TIA1/4724608 miR-19b can also regulate TIA1 expression in CRC. (A) Schematic description of the hypothetical duplex formed by the interaction between the binding site in the TIA1 3’-UTR and miR-19b. The miR-19b seed sequence and the seed sequence binding sites in the TIA1 3’-UTR are indicated in red. All nucleotides of the seed sequence of the binding site are conserved in several species, including human, mouse, rat and rabbit. The predicted free energy values of the hybrids are indicated. (B) Quantitative RT-PCR analysis of miR-19b expression levels in the same 16 pairs of CRC and normal tissue samples. (C) Pearson’s correlation scatter plot of the fold change of miR-19b and TIA1 protein in human CRC tissue pairs. (D) Quantitative RT-PCR analysis of miR-19b levels in SW480 cells transfected with control mimic, miR-19b mimic, control inhibitor or miR-19b inhibitor. (E and F) Western blot analysis of TIA1 protein levels in SW480 cells transfected with control mimic, miR-19b mimic, control inhibitor or miR-19b inhibitor. E: representative images; F: quantitative analysis. ***P < 0.001. (TIF 917 kb)