10.6084/m9.figshare.c.3701899_D3.v1 Benjamin Kidder Benjamin Kidder Gangqing Hu Gangqing Hu Kairong Cui Kairong Cui Keji Zhao Keji Zhao MOESM10 of SMYD5 regulates H4K20me3-marked heterochromatin to safeguard ES cell self-renewal and prevent spurious differentiation Springer Nature 2017 Embryonic stem cells SMYD5 H4K20me3 Repetitive DNA LTR LINE Pluripotent Epigenetics Chromatin Heterochromatin Genomics RNA-Seq ChIP-Seq Self-renewal Gene expression Embryoid body Differentiation Histone methyltransferase 2017-02-23 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM10_of_SMYD5_regulates_H4K20me3-marked_heterochromatin_to_safeguard_ES_cell_self-renewal_and_prevent_spurious_differentiation/4689247 Additional file 10: Figure S10. Decreased H3K9me3 heterochromatin foci in SMYD5-depleted ES cells. (A, B) Immunofluorescence staining of H3K9me3 in (A) shLuc and (B) shSmyd5 ES cells. Nuclei were stained with DAPI. (C) Quantitation of nuclear H3K9me3 heterochromatin foci using ImageJ software.