Additional file 3: Figure S3. of Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation Hao Feng Ilmari Pyykkö Jing Zou 10.6084/m9.figshare.c.3698866_D4.v1 https://springernature.figshare.com/articles/figure/Additional_file_3_Figure_S3_of_Involvement_of_Ubiquitin-Editing_Protein_A20_in_Modulating_Inflammation_in_Rat_Cochlea_Associated_with_Silver_Nanoparticle-Induced_CD68_Upregulation_and_TLR4_Activation/4675678 MCP2+ cells in the rat cochlea 7 days post-intratympanic injection of 0.4 % AgNPs shown by immunofluorescence confocal microscopy or immunohistochemistry. In the cochleae exposed to dH2O, the strial basal cells (SBCs), spiral ligament fibrocytes (SLFs), spiral ganglion cells (SGCs), and hair cells (HCs), pillar cells (PCs), and Deiters’ cells (DCs) of Corti’s organ (CO) showed intensive staining (B, D, and F). 0.4 % AgNPs had no influence on the staining in the SBCs, SLFs, SGCs, and CO (A, C, and E). Comparisons of staining intensity are shown in G and H. Scale bar = 50 μm in A–D and 20 μm in E and F. (JPG 4614 kb) 2016-05-04 05:00:00 rat cochlea 7  days post-intratympanic injection hair cells dH 2O HC JPG 4614 kb strial basal cells PC Ubiquitin-Editing Protein 20 μ 20 μ m TLR CO Rat Cochlea Associated immunofluorescence confocal microscopy SBC DC Silver Nanoparticle-Induced CD 68 Upregulation SGC SLF Modulating Inflammation staining intensity MCP