10.6084/m9.figshare.c.3698830_D1.v1 M. Meckel M. Meckel R. Bergmann R. Bergmann M. Miederer M. Miederer F. Roesch F. Roesch Additional file 1: of Bone targeting compounds for radiotherapy and imaging: *Me(III)-DOTA conjugates of bisphosphonic acid, pamidronic acid and zoledronic acid Springer Nature 2016 Hydroxy bisphosphonates PET Theranostics Bone metastases 68Ga 177Lu DOTA Zoledronic acid 2016-09-23 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Bone_targeting_compounds_for_radiotherapy_and_imaging_Me_III_-DOTA_conjugates_of_bisphosphonic_acid_pamidronic_acid_and_zoledronic_acid/4675570 Figure S1. Quality control of [68Ga]DOTAZOL by means of cross checked radio-HPLC and radio-TLC, as a representative of 68Ga-labelled bisphosphonates. A: After 15 min reaction time. B: After SPE purification. A(HPLC): Rt([68Ga]DOTAZOL) = 0.6 min. (88 %), Rt([68Ga]DFO) = 4.1 min. (12 %). B(HPLC): Rt([68Ga]DOTAZOL) = 0.6 min. (98 %). A(TLC): Rf([68Ga]DOTAZOL) = 0.1 (85 %), Rf([68Ga]acetylacetonate) = 0.9 (15 %). B(TLC): Rf([68Ga]DOTAZOL) = 0.1 (99 %). Control(TLC): Rf([68Ga] acetylacetonate) = 0.9 (99 %). Figure S2. Radio-HPLC of [177Lu]DOTAZOL on a Zorbax 300SB-C18 9,4 × 250 mm 5 μ, A = 100 mM TEAP pH = 2,24, isocratic flow: 1 ml/min. Figure S3. Radio-HPLC of urine and blood samples after administration of [177Lu]DOTAZOL. The HPLC method used for analysing the Lu-177 complexes is also suitable for the Ga-68 complexes of DOTA-Bisphosphonates. The Ga-68 complexes showed a different retention time based on the different character of the DOTA complex. This is also known for other DOTA-Compounds like DOTATOC or DOTATATE. Figure S4. Radio-HPLC of [68Ga]DOTAZOL on a Zorbax 300SB-C18 9,4 × 250 mm 5 μ, A = 100 mM TEAP pH = 2,24, isocratic flow: 1 ml/min. (DOCX 742 kb)