Additional file 5: Figure S5. of Blockade of autophagy reduces pancreatic cancer stem cell activity and potentiates the tumoricidal effect of gemcitabine Ming-Chen Yang Hao-Chen Wang Ya-Chin Hou Hui-Ling Tung Tai-Jan Chiu Yan-Shen Shan 10.6084/m9.figshare.c.3697834_D1.v1 https://springernature.figshare.com/articles/figure/Additional_file_5_Figure_S5_of_Blockade_of_autophagy_reduces_pancreatic_cancer_stem_cell_activity_and_potentiates_the_tumoricidal_effect_of_gemcitabine/4673668 OPN triggered STAT3, ERK, and NF-κB activation, but not AKT, JNK, and p38 MAPK in pancreatic cancer cells. (A) PANC-1 and MIA PaCa-2 cells were treated with OPN (100 ng/mL) for the indicated time points. The cell lysates were prepared and subjected to Western blotting using the indicated antibodies. (B) PANC-1 and MIA PaCa-2 cells were incubated with OPN (100 ng/mL) for the indicated time points. Cells were lysed and fractionated, and the levels of NF-κB p65 in both cytosolic and nuclear fractions were detected by Western blotting. β-Actin serves as a cytosolic marker, and LAMIN A/C serves as a nuclear marker. (For detail, please see Additional file 8). (TIFF 2417 kb) 2015-10-12 05:00:00 ALDH1 Autophagy Cancer stem cells CD133 CD44 Gemcitabine Osteopontin Pancreatic cancer