%0 Journal Article %A Kumar, Dilip %A Puan, Kia %A Andiappan, Anand %A Lee, Bernett %A Westerlaken, Geertje %A Haase, Doreen %A Melchiotti, Rossella %A Li, Zhuang %A Yusof, Nurhashikin %A Lum, Josephine %A Koh, Geraldine %A Foo, Shihui %A Yeong, Joe %A Alves, Alexessander %A Pekkanen, Juha %A Sun, Liang %A Irwanto, Astrid %A Fairfax, Benjamin %A Naranbhai, Vivek %A Common, John %A Tang, Mark %A Chuang, Chin %A Jarvelin, Marjo-Riitta %A Knight, Julian %A Zhang, Xuejun %A Chew, Fook %A Prabhakar, Shyam %A Jianjun, Liu %A Yun Wang, De %A Zolezzi, Francesca %A Poidinger, Michael %A Lane, E. %A Meyaard, Linde %A Rötzschke, Olaf %D 2017 %T Additional file 8: of A functional SNP associated with atopic dermatitis controls cell type-specific methylation of the VSTM1 gene locus %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_8_of_A_functional_SNP_associated_with_atopic_dermatitis_controls_cell_type-specific_methylation_of_the_VSTM1_gene_locus/4670119 %R 10.6084/m9.figshare.c.3696271_D8.v1 %2 https://springernature.figshare.com/ndownloader/files/7623337 %K VSTM1 %K Signal inhibitory receptor on leukocytes-1 (SIRL-1) %K Expression quantitative trait loci (eQTL) %K Atopic dermatitis %K Monocytes %K Reactive oxygen species (ROS) %K Neutrophils %X Model on the potentiating effect of a regulatory CpG-SNP. A Conventional vs. regulatory CpG-SNPs. The methylation of CpG pairs results in the condensation of chromatin due to a tighter packaging of the histone-DNA complexes (open circle: non-methylated CpG, closed circle: methylated CpG). While a conventional CpG-SNP can assist this process by providing only one additional allele-dependent methylation site (middle panel), a regulatory CpG-SNP (such as rs612529) modulates the recruiting of CpG-modifying enzymes (right panel). In the case of rs612529, this seems to be mediated by the allele-specific binding of PU.1 to the T allele of the CpG-SNP. In monocytes PU.1 was reported to bind the demethylase Tet-2. B Potentiating effect. Recruitment of Tet-2 mediates the chromatin-opening by facilitating the demethylation of all CpG pairs in the vicinity. The epigenetic effect is allele-specific (T allele) and direction of the direction probably cell-type–dependent. (PDF 415 kb) %I figshare