%0 Journal Article %A Schöfl, Gerhard %A Lang, Kathrin %A Quenzel, Philipp %A Böhme, Irina %A Sauter, Jürgen %A Hofmann, Jan %A Pingel, Julia %A Schmidt, Alexander %A Lange, Vinzenz %D 2017 %T Additional file 4: Figure S3. of 2.7 million samples genotyped for HLA by next generation sequencing: lessons learned %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_Figure_S3_of_2_7_million_samples_genotyped_for_HLA_by_next_generation_sequencing_lessons_learned/4653130 %R 10.6084/m9.figshare.c.3691699_D1.v1 %2 https://springernature.figshare.com/ndownloader/files/7587097 %K Next generation sequencing %K HLA genotyping %K High resolution %K High throughput %K Amplicon PCR %K DKMS %K Primer dimers %K PCR chimerism %K Novel alleles %X Average primer dimer rates for different amplicons, genes, PCR setups and reaction volumes. PCR setups are a 1-PCR 4-primer setup vs. a standard 2-PCR 2-primer setup. Differences in reaction volumes are denoted by PCR (10 μl) and Fluidigm (10 nl). Only amplicons with an average PD rate > 0.2% are labelled in the plot. The y-axis is square root scaled to enhance readability. (PDF 5 kb) %I figshare