10.6084/m9.figshare.c.3665815_D6.v1
Yipeng Wang
Yipeng
Wang
Varun Balaji
Varun
Balaji
Senthilvelrajan Kaniyappan
Senthilvelrajan
Kaniyappan
Lars Krüger
Lars
Krüger
Stephan Irsen
Stephan
Irsen
Katharina Tepper
Katharina
Tepper
RamReddy Chandupatla
RamReddy
Chandupatla
Walter Maetzler
Walter
Maetzler
Anja Schneider
Anja
Schneider
Eckhard Mandelkow
Eckhard
Mandelkow
Eva-Maria Mandelkow
Eva-Maria
Mandelkow
Additional file 3: Figure S3. of The release and trans-synaptic transmission of Tau via exosomes
Springer Nature
2017
Tau
Alzheimer disease
Exosomes
Spreading
2017-01-13 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_3_Figure_S3_of_The_release_and_trans-synaptic_transmission_of_Tau_via_exosomes/4552531
Projections of neurons cultured in microfluidic devices with short microgrooves (150 μm). The 1st order hippocampal neurons were seeded on the somal side (left) of the microfluidic chamber. Fourteen days later, the 2nd order hippocampal neurons were seeded on the neuritic side (right) and cultured for additional 10–11 days. Neurons were treated on the neuritic side (A) or somal side (C) with DiI for 2 to 3 h. The living cells were then imaged using fluorescence microscopy. Scale bar = 10 μm. When the 2nd order neurons on the neuritic side were treated with DiI (A, top right panel), cell bodies of the 1st order neurons whose neurites projected through microgrooves to the neuritic side were positive for DiI staining (arrowhead in A, top left panel), by contrast, cell bodies of neurons that do not project to the neuritic side were not positive for DiI staining (arrows in A, bottom left panel). When the 1st order neurons on the somal side were treated with DiI (C, top left panel), their processes that projected through microgrooves to the neuritic side were stained by DiI (C, top right panel). However, no cell bodies of the 2nd order neurons (arrows in C, bottom right) were positive for DiI staining, indicating that the 2nd order neurons do not project through microgrooves to the somal side. The result of A and C is illustrated by B and D resp.. The flow of the conditioned medium (indicated by arrow) prevents the diffusion of added Dil from treated side to the opposite side. (PNG 175 kb)