10.6084/m9.figshare.c.3663442_D2.v1 Zsigmond Benko Zsigmond Benko Dong Liang Dong Liang Ge Li Ge Li Robert Elder Robert Elder Anindya Sarkar Anindya Sarkar Jun Takayama Jun Takayama Arun Ghosh Arun Ghosh Richard Zhao Richard Zhao MOESM1 of A fission yeast cell-based system for multidrug resistant HIV-1 proteases Springer Nature 2017 HIV-1 Multidrug resistant proteases Fission yeast Proteolytic cleavage Cell proliferation Oxidative stress Mitochondria Cell death Protease inhibitors 2017-01-11 05:00:00 Presentation https://springernature.figshare.com/articles/presentation/MOESM1_of_A_fission_yeast_cell-based_system_for_multidrug_resistant_HIV-1_proteases/4541938 Additional file 1: Figure S1. DRV and its derivatives suppress the M7PR but not the M10PR or M11PR over time. The chemical structures of protease inhibitors, DRV, UIC-94003, GRL-0489A, GRL-0249A, GRL-0159A and GRL-044-10A are shown in (Fig. 4A). All six compounds including DRV are P2 ligands [17]. Effects of the newly synthesized protease inhibitors on mdrPR-induced growth arrest were measured against the wtPR and mdrPRs by using a liquid growth assay and measured by OD650 over a time period from 16 to 72 h after the drug treatments and gene inductions. The final drug concentration of 200 µM was used in each of the experiments. DRV was used here as a positive control and no drug treatment was used as a negative control.