%0 Journal Article %A Liu, Qian %A Gao, Ranran %A Li, Jingen %A Lin, Liangcai %A Zhao, Junqi %A Sun, Wenliang %A Tian, Chaoguang %D 2017 %T MOESM4 of Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering %U https://springernature.figshare.com/articles/journal_contribution/MOESM4_of_Development_of_a_genome-editing_CRISPR_Cas9_system_in_thermophilic_fungal_Myceliophthora_species_and_its_application_to_hyper-cellulase_production_strain_engineering/4516220 %R 10.6084/m9.figshare.c.3656969_D4.v1 %2 https://springernature.figshare.com/ndownloader/files/7302362 %K Myceliophthora %K CRISPR/Cas9 system %K Thermophilic fungi %K Cellulases %X Additional file 4: Figure S4. Verification of triple-gene deletions of cre-1, res-1, gh1-1 and alp-1 in selected transformants. (A) Schematic of homologous recombination (HR) of cre-1, res-1 and gh1-1 mediated by Cas9, sgRNAs and donor DNA. (B) PCR analysis of triple-gene deletion of cre-1, res-1 and gh1-1 in selected transformants with specific paired primers (cre1/gh1-1/res1-out-F and cre1/gh1-1/res1-in-R). The expected length of disrupted transformants was 1.9 kb, while that of the host strain (rightmost lane) was 1.0 kb. %I figshare