Additional file 4: Figure S4. of Agonists and knockdown of estrogen receptor β differentially affect invasion of triple-negative breast cancer cells in vitro Susanne Schüler-Toprak Julia Häring Elisabeth Inwald Christoph Moehle Olaf Ortmann Oliver Treeck 10.6084/m9.figshare.c.3650744_D2.v1 https://springernature.figshare.com/articles/figure/Additional_file_4_Figure_S4_of_Agonists_and_knockdown_of_estrogen_receptor_differentially_affect_invasion_of_triple-negative_breast_cancer_cells_in_vitro/4491062 Proliferation of MDA-MB-231 and HS578T breast cancer cells after knockdown of ESR2 gene expression. The day after transfection with negative control siRNA or ESR2 siRNA, cells were seeded in DMEM/F12 plus 10% FSC in triplicates and relative numbers of viable cells were measured on day 0, 2, 3 and 4 using the Cell Titer Blue assay (Promega) as described in the Materials and Methods section. Values are expressed in percent of proliferation of mock-transfected cells (n = 3). *p < 0.05 vs. mock-transfected cells (Kruskal-Wallis H-test with Bonferroni post-hoc test). (JPG 41 kb) 2016-12-21 05:00:00 Estrogen receptor beta Triple-negative breast cancer Cell culture Invasion