Boradia, Vishant Patil, Pravinkumar Agnihotri, Anushri Kumar, Ajay Rajwadi, Kalpesh Sahu, Ankit Bhagath, Naveen Sheokand, Navdeep Kumar, Manoj Malhotra, Himanshu Patkar, Rachita Hasan, Navi Raje, Manoj Raje, Chaaya MOESM2 of Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv Additional file 1: Figure 2 Intact mass analysis and isoelectric point of recombinant M.tb GAPDH. MALDI-TOF confirms the presence of a single protein corresponding to a. MT-GAPDH-H and b. EC-H-GAPDH. Two dimensional gel analysis of rGAPDH purified from different source c. MT-rGAPDH d. MS-rGAPDH e. EC-rGAPDH where the gel was either stained with coomassie or western blot using rabbit α-GAPDH or mouse α-His. f. Peptide mass fingerprinting (PMF) of ~ 50 kD M. smegmatis protein that co-elutes with rPykA. Identified sequences are indicated in bold face. Identified sequences;EC-H-GAPDH;MS-rGAPDH e;Peptide mass fingerprinting;Mycobacterium tuberculosis H 37Ra;MT-GAPDH-H;isoelectric point;multimeric proteins;PMF;M.tb GAPDH;smegmatis protein;MOESM 2;MALDI-TOF;rabbit α- GAPDH;M.tb H 37Rv;gel analysis;source c;mouse α- 2016-08-11
    https://springernature.figshare.com/articles/figure/MOESM2_of___Mycobacterium_tuberculosis_H37Ra_a_surrogate_for_the_expression_of_conserved_multimeric_proteins_of_M_tb_H37Rv/4475552
10.6084/m9.figshare.c.3646511_D4.v1