%0 Figure %A Boradia, Vishant %A Patil, Pravinkumar %A Agnihotri, Anushri %A Kumar, Ajay %A Rajwadi, Kalpesh %A Sahu, Ankit %A Bhagath, Naveen %A Sheokand, Navdeep %A Kumar, Manoj %A Malhotra, Himanshu %A Patkar, Rachita %A Hasan, Navi %A Raje, Manoj %A Raje, Chaaya %D 2016 %T MOESM2 of Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv %U https://springernature.figshare.com/articles/figure/MOESM2_of___Mycobacterium_tuberculosis_H37Ra_a_surrogate_for_the_expression_of_conserved_multimeric_proteins_of_M_tb_H37Rv/4475552 %R 10.6084/m9.figshare.c.3646511_D4.v1 %2 https://springernature.figshare.com/ndownloader/files/7205453 %K Identified sequences %K EC-H-GAPDH %K MS-rGAPDH e %K Peptide mass fingerprinting %K Mycobacterium tuberculosis H 37Ra %K MT-GAPDH-H %K isoelectric point %K multimeric proteins %K PMF %K M.tb GAPDH %K smegmatis protein %K MOESM 2 %K MALDI-TOF %K rabbit α- GAPDH %K M.tb H 37Rv %K gel analysis %K source c %K mouse α- %X Additional file 1: Figure 2 Intact mass analysis and isoelectric point of recombinant M.tb GAPDH. MALDI-TOF confirms the presence of a single protein corresponding to a. MT-GAPDH-H and b. EC-H-GAPDH. Two dimensional gel analysis of rGAPDH purified from different source c. MT-rGAPDH d. MS-rGAPDH e. EC-rGAPDH where the gel was either stained with coomassie or western blot using rabbit α-GAPDH or mouse α-His. f. Peptide mass fingerprinting (PMF) of ~ 50 kD M. smegmatis protein that co-elutes with rPykA. Identified sequences are indicated in bold face. %I figshare