Guillotte, Micheline Nato, Farida Juillerat, Alexandre Hessel, Audrey Marchand, Françoise Lewit-Bentley, Anita Bentley, Graham Vigan-Womas, Inès Mercereau-Puijalon, Odile MOESM2 of Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin Additional file 2: Table S1. Summary table of competition ELISAs. Competition ELISA were carried out as described in section “Methods”. In brief, saturating concentrations of unlabelled monoclonal D15-50, D15-68, E20-76, BD20E4, BDEE10, M21-17 and M21-30 IgG were incubated with eDBL1-coated plates for 2 h at 37 ℃, unbound IgG were washed out and biotinylated D15-50, E20-76 or BD20E4 IgG were added to individual wells at a concentration previously determined to generate a signal of approximately 1 OD after incubation at 4 ℃ for 20 min. Binding of the biotin-labelled IgG was monitored using streptavidin-labelled horseradish peroxidase. Malaria;Rosetting;PfEMP1 adhesin;Monoclonal antibodies (mAbs);Rosette-disrupting antibodies;Epitopes;Antigenicity 2016-01-15
    https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_Functional_analysis_of_monoclonal_antibodies_against_the_Plasmodium_falciparum_PfEMP1-VarO_adhesin/4470956
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