10.6084/m9.figshare.c.3645116_D4.v1
Micheline Guillotte
Micheline
Guillotte
Farida Nato
Farida
Nato
Alexandre Juillerat
Alexandre
Juillerat
Audrey Hessel
Audrey
Hessel
Françoise Marchand
Françoise
Marchand
Anita Lewit-Bentley
Anita
Lewit-Bentley
Graham Bentley
Graham
Bentley
Inès Vigan-Womas
Inès
Vigan-Womas
Odile Mercereau-Puijalon
Odile
Mercereau-Puijalon
MOESM2 of Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin
Springer Nature
2016
Malaria
Rosetting
PfEMP1 adhesin
Monoclonal antibodies (mAbs)
Rosette-disrupting antibodies
Epitopes
Antigenicity
2016-01-15 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_Functional_analysis_of_monoclonal_antibodies_against_the_Plasmodium_falciparum_PfEMP1-VarO_adhesin/4470956
Additional file 2: Table S1. Summary table of competition ELISAs. Competition ELISA were carried out as described in section “Methods”. In brief, saturating concentrations of unlabelled monoclonal D15-50, D15-68, E20-76, BD20E4, BDEE10, M21-17 and M21-30 IgG were incubated with eDBL1-coated plates for 2 h at 37 ℃, unbound IgG were washed out and biotinylated D15-50, E20-76 or BD20E4 IgG were added to individual wells at a concentration previously determined to generate a signal of approximately 1 OD after incubation at 4 ℃ for 20 min. Binding of the biotin-labelled IgG was monitored using streptavidin-labelled horseradish peroxidase.