%0 Journal Article %A Niwa, Hitoshi %A Nakamura, Akira %A Urata, Makoto %A Shirae-Kurabayashi, Maki %A Kuraku, Shigehiro %A Russell, Steven %A Ohtsuka, Satoshi %D 2016 %T Additional file 1: of The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_The_evolutionally-conserved_function_of_group_B1_Sox_family_members_confers_the_unique_role_of_Sox2_in_mouse_ES_cells/4469804 %R 10.6084/m9.figshare.c.3644711_D2.v1 %2 https://springernature.figshare.com/ndownloader/files/7199366 %K Pluripotent stem cells %K Sox2 %K Evolution %K Co-option %X Figure S1. Analysis of Sox2-null ES cells rescued by wild-type Sox factors. A Morphology of primary transfectants with Sox6 and Sox7. Transfection of Sox6 expression vectors caused differentiation of large flat trophectoderm-like cells, whereas with Sox7 spindle cells resembling parietal endoderm were occasionally observed. B Immunostaining of Sox2-null ES cells rescued by Sox1 with anti-Sox1. Ectopic expression of Sox1 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. C Immunostaining of Sox2-null ES cells rescued by Sox3 with anti-Sox3. Ectopic expression of Sox3 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. D Immunostaining of Sox2-null ES cells rescued by Sox15 with anti-Sox15. Ectopic expression of Sox15 in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control. (PDF 2664 kb) %I figshare