10.6084/m9.figshare.c.3643841_D1.v1
Yan Li
Yan
Li
Wei Zhang
Wei
Zhang
Jianxin Gao
Jianxin
Gao
Jiaqi Liu
Jiaqi
Liu
Hongtao Wang
Hongtao
Wang
Jun Li
Jun
Li
Xuekang Yang
Xuekang
Yang
Ting He
Ting
He
Hao Guan
Hao
Guan
Zhao Zheng
Zhao
Zheng
Shichao Han
Shichao
Han
Maolong Dong
Maolong
Dong
Juntao Han
Juntao
Han
Jihong Shi
Jihong
Shi
Dahai Hu
Dahai
Hu
Additional file 2: Figure S3. of Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway
Springer Nature
2016
ADSC-CM
Hypertrophic scars
Myofibroblasts
p38 pathway
Collagen
α-SMA
2016-08-02 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S3_of_Adipose_tissue-derived_stem_cells_suppress_hypertrophic_scar_fibrosis_via_the_p38_MAPK_signaling_pathway/4466915
The effect of ADSC-CM on the primary NS-derived fibroblasts in vitro. (A-C) qRT-PCR analysis for the mRNA levels of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph representeds the expression of Col1, Col3, and α-SMA relative to that of GAPDH. (D–G) Immunoblot analysis for the protein expression of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph shows the relative band density to β-actin. (H, I) Immunoblot analysis for the expression of p-p38 stimulated for 1 h with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %). Every experiment was repeated three times at least. The data are shown as mean ± SEM from three independent experiments (*P < 0.05; **P < 0.01). (TIF 1346 kb)