10.6084/m9.figshare.c.3643841_D1.v1 Yan Li Yan Li Wei Zhang Wei Zhang Jianxin Gao Jianxin Gao Jiaqi Liu Jiaqi Liu Hongtao Wang Hongtao Wang Jun Li Jun Li Xuekang Yang Xuekang Yang Ting He Ting He Hao Guan Hao Guan Zhao Zheng Zhao Zheng Shichao Han Shichao Han Maolong Dong Maolong Dong Juntao Han Juntao Han Jihong Shi Jihong Shi Dahai Hu Dahai Hu Additional file 2: Figure S3. of Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway Springer Nature 2016 ADSC-CM Hypertrophic scars Myofibroblasts p38 pathway Collagen α-SMA 2016-08-02 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S3_of_Adipose_tissue-derived_stem_cells_suppress_hypertrophic_scar_fibrosis_via_the_p38_MAPK_signaling_pathway/4466915 The effect of ADSC-CM on the primary NS-derived fibroblasts in vitro. (A-C) qRT-PCR analysis for the mRNA levels of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph representeds the expression of Col1, Col3, and α-SMA relative to that of GAPDH. (D–G) Immunoblot analysis for the protein expression of Col1, Col3, and α-SMA in NSFs stimulated with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %) for 24 h; graph shows the relative band density to β-actin. (H, I) Immunoblot analysis for the expression of p-p38 stimulated for 1 h with different concentrations of ADSC-CM (10 %, 20 %, 40 %, 80 %). Every experiment was repeated three times at least. The data are shown as mean ± SEM from three independent experiments (*P < 0.05; **P < 0.01). (TIF 1346 kb)