Additional file 4: of Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength Vihandha Wickramasinghe Mar Gonzàlez-Porta David Perera Arthur Bartolozzi Christopher Sibley Martina Hallegger Jernej Ule John Marioni Ashok Venkitaraman 10.6084/m9.figshare.c.3643007_D2.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_4_of_Regulation_of_constitutive_and_alternative_mRNA_splicing_across_the_human_transcriptome_by_PRPF8_is_determined_by_5_splice_site_strength/4464089 Validation of mitotic arrest following PRPF8 depletion with two independent siRNAs. a U2OS osteosarcoma cells depleted of PRPF8 using two independent siRNAs and monitored by phase-contrast time-lapse microscopy spend significantly longer in mitosis (measured from nuclear envelope breakdown (NEB) to chromatin decondensation), as opposed to control cells, in which mitosis lasts, on average, <60 min. Statistically significant pairwise comparisons are shown (***p < 0.001). b Introduction of properly spliced and processed mRNA into Control siRNA-treated cells has no effect on mitotic index. Control siRNA-treated cells were transfected with properly spliced and processed mRNA purified from whole cells and analyzed for mitotic index. Plots represent the mean from two independent experiments ± standard error of the mean. n.s. not significant. (PDF 77 kb) 2015-09-21 05:00:00 mRNA NEB Control siRNA-treated cells PRPF 8 PRPF 8 depletion mitosi control siRNA-treated cells phase-contrast time-lapse microscopy PDF 77 kb mitotic index U 2OS osteosarcoma cells