%0 Journal Article %A Chen, Shilong %A Luo, Guifeng %A Yang, Zhou %A Lin, Shuncheng %A Chen, Shaoying %A Wang, Song %A Goraya, Mohsan %A Chi, Xiaojuan %A Zeng, Xiancheng %A Chen, Ji-Long %D 2016 %T MOESM7 of Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways %U https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_Avian_Tembusu_virus_infection_effectively_triggers_host_innate_immune_response_through_MDA5_and_TLR3-dependent_signaling_pathways/4464083 %R 10.6084/m9.figshare.c.3643004_D8.v1 %2 https://springernature.figshare.com/ndownloader/files/7193510 %K 36 h %K 293 T cells %K IFN %K Avian Tembusu virus infection %K RT-PCR %K MDA %K TLR %K NF -κB activation %K IRF %K IL -29. C %K MOESM %K -28A %K ATMUV %K DMSO %X Additional file 7 IRF3, IRF7 and NF-κB activation are required for efficient expression of IFN induced by ATMUV. A and B. IRF3, IRF7 knockdown 293T cell lines or luciferase control were infected with or without ATMUV for 36 h. RT-PCR were performed to measure the interference efficiency of IRF3, IRF7 and the production of IFN-β, IL-28A/B and IL-29. C. 293T cells were treated with BAY11-7082 (5 µM) or DMSO for 3 h, followed by ATMUV infection for 36 h. The cells were harvested and the mRNA levels of IFN-β, IL-28A/B and IL-29 were analyzed by RT-PCR. %I figshare