%0 Journal Article %A Thrane, Susan %A Janitzek, Christoph %A Matondo, Sungwa %A Resende, Mafalda %A Gustavsson, Tobias %A Jongh, Willem %A Clemmensen, Stine %A Roeffen, Will %A Vegte‑Bolmer, Marga %A Gemert, Geert %A Sauerwein, Robert %A Schiller, John %A Nielsen, Morten %A Theander, Thor %A Salanti, Ali %A Sander, Adam %D 2016 %T MOESM5 of Bacterial superglue enables easy development of efficient virus-like particle based vaccines %U https://springernature.figshare.com/articles/journal_contribution/MOESM5_of_Bacterial_superglue_enables_easy_development_of_efficient_virus-like_particle_based_vaccines/4463369 %R 10.6084/m9.figshare.c.3642749_D5.v1 %2 https://springernature.figshare.com/ndownloader/files/7192787 %K 5 µ g antigen %K Anti-IL %K ELISA %K 180 binding motifs %K BALB %K IL %K IgG titers %K 3 rd immunization %K 2 xSpyTag-VLP vaccine %K MOESM %K 2 xSpyTag-VLP vaccines %X Additional file 5: Figure S4. Comparison of the immunogenicity of IL-5 SpyTag-VLP and IL-5 2xSpyTag-VLP vaccines. BALB/c mice were immunized with 5 µg antigen (SpyCatcher-IL-5) mixed with either SpyTag-VLP with 180 binding motifs (n = 4, open squares) or 2xSpyTag-VLP (n = 5, filled circles) with 360 potential binding motifs. Anti-IL-5 IgG titers were determined by standard ELISA using sera obtained two weeks the 3rd immunization. Anti-IL-5 IgG titers were higher in mice immunized with the 2xSpyTag-VLP vaccine (filled circles) compared to the SpyTag-VLP vaccine (open squares). The difference approached statistical significance (P = 0.06, Mann–Whitney Rank Sum test). %I figshare