10.6084/m9.figshare.c.3642749_D5.v1 Susan Thrane Susan Thrane Christoph Janitzek Christoph Janitzek Sungwa Matondo Sungwa Matondo Mafalda Resende Mafalda Resende Tobias Gustavsson Tobias Gustavsson Willem Jongh Willem Jongh Stine Clemmensen Stine Clemmensen Will Roeffen Will Roeffen Marga Vegte‑Bolmer Marga Vegte‑Bolmer Geert Gemert Geert Gemert Robert Sauerwein Robert Sauerwein John Schiller John Schiller Morten Nielsen Morten Nielsen Thor Theander Thor Theander Ali Salanti Ali Salanti Adam Sander Adam Sander MOESM5 of Bacterial superglue enables easy development of efficient virus-like particle based vaccines Springer Nature 2016 5 µ g antigen Anti-IL ELISA 180 binding motifs BALB IL IgG titers 3 rd immunization 2 xSpyTag-VLP vaccine MOESM 2 xSpyTag-VLP vaccines 2016-04-27 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM5_of_Bacterial_superglue_enables_easy_development_of_efficient_virus-like_particle_based_vaccines/4463369 Additional file 5: Figure S4. Comparison of the immunogenicity of IL-5 SpyTag-VLP and IL-5 2xSpyTag-VLP vaccines. BALB/c mice were immunized with 5 µg antigen (SpyCatcher-IL-5) mixed with either SpyTag-VLP with 180 binding motifs (n = 4, open squares) or 2xSpyTag-VLP (n = 5, filled circles) with 360 potential binding motifs. Anti-IL-5 IgG titers were determined by standard ELISA using sera obtained two weeks the 3rd immunization. Anti-IL-5 IgG titers were higher in mice immunized with the 2xSpyTag-VLP vaccine (filled circles) compared to the SpyTag-VLP vaccine (open squares). The difference approached statistical significance (P = 0.06, Mann–Whitney Rank Sum test).