10.6084/m9.figshare.c.3642749_D5.v1
Susan Thrane
Susan
Thrane
Christoph Janitzek
Christoph
Janitzek
Sungwa Matondo
Sungwa
Matondo
Mafalda Resende
Mafalda
Resende
Tobias Gustavsson
Tobias
Gustavsson
Willem Jongh
Willem
Jongh
Stine Clemmensen
Stine
Clemmensen
Will Roeffen
Will
Roeffen
Marga Vegte‑Bolmer
Marga
Vegte‑Bolmer
Geert Gemert
Geert
Gemert
Robert Sauerwein
Robert
Sauerwein
John Schiller
John
Schiller
Morten Nielsen
Morten
Nielsen
Thor Theander
Thor
Theander
Ali Salanti
Ali
Salanti
Adam Sander
Adam
Sander
MOESM5 of Bacterial superglue enables easy development of efficient virus-like particle based vaccines
Springer Nature
2016
5 µ g antigen
Anti-IL
ELISA
180 binding motifs
BALB
IL
IgG titers
3 rd immunization
2 xSpyTag-VLP vaccine
MOESM
2 xSpyTag-VLP vaccines
2016-04-27 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM5_of_Bacterial_superglue_enables_easy_development_of_efficient_virus-like_particle_based_vaccines/4463369
Additional file 5: Figure S4. Comparison of the immunogenicity of IL-5 SpyTag-VLP and IL-5 2xSpyTag-VLP vaccines. BALB/c mice were immunized with 5 µg antigen (SpyCatcher-IL-5) mixed with either SpyTag-VLP with 180 binding motifs (n = 4, open squares) or 2xSpyTag-VLP (n = 5, filled circles) with 360 potential binding motifs. Anti-IL-5 IgG titers were determined by standard ELISA using sera obtained two weeks the 3rd immunization. Anti-IL-5 IgG titers were higher in mice immunized with the 2xSpyTag-VLP vaccine (filled circles) compared to the SpyTag-VLP vaccine (open squares). The difference approached statistical significance (P = 0.06, Mann–Whitney Rank Sum test).