%0 Journal Article %A Thrane, Susan %A Janitzek, Christoph %A Matondo, Sungwa %A Resende, Mafalda %A Gustavsson, Tobias %A Jongh, Willem %A Clemmensen, Stine %A Roeffen, Will %A Vegte‑Bolmer, Marga %A Gemert, Geert %A Sauerwein, Robert %A Schiller, John %A Nielsen, Morten %A Theander, Thor %A Salanti, Ali %A Sander, Adam %D 2016 %T MOESM3 of Bacterial superglue enables easy development of efficient virus-like particle based vaccines %U https://springernature.figshare.com/articles/journal_contribution/MOESM3_of_Bacterial_superglue_enables_easy_development_of_efficient_virus-like_particle_based_vaccines/4463360 %R 10.6084/m9.figshare.c.3642749_D2.v1 %2 https://springernature.figshare.com/ndownloader/files/7192778 %K SDS-PAGE %K SpyTag %K antigen %K PD-L 1 %K Pf %K 12  h %K Bacterial superglue %K VAR 2CSA Reactions %K Ag 85A %K HER %K 2. %K SpyCatcher %K MOESM 3 %K spy-VLP %K Survivin %K efficiency %K CTLA %K IL %K CIDR %K CSP %K vaccine %K Figure %K PCSK %K virus-like particle %K 4 OC %X Additional file 3: Figure S2. Estimated coupling efficiencies of spy-VLP/antigen reactions. SDS-PAGE gels demonstrating that SpyTag or SpyCatcher fused antigens had reacted with corresponding spy-VLPs. (A) Survivin, (B) CIDR, (C) IL-5, (D) Pfs25, (E) Ag85A, (F) CSP, (G) HER2, (H) PCSK9, (I) CTLA-4, (J) PD-L1 and (K) VAR2CSA. Reactions were incubated for at least 12 h at 4OC. %I figshare