%0 Figure %A Huang, Nick %A Lou, Mabel %A Liu, Hua %A Avila, Cecilia %A Ma, Yupo %D 2016 %T Additional file 2: Figure S2. of Identification of a potent small molecule capable of regulating polyploidization, megakaryocyte maturation, and platelet production %U https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_Identification_of_a_potent_small_molecule_capable_of_regulating_polyploidization_megakaryocyte_maturation_and_platelet_production/4462478 %R 10.6084/m9.figshare.c.3642425_D1.v1 %2 https://springernature.figshare.com/ndownloader/files/7191893 %K BM %K SCF %K 4 %K HSC %K TPO %K CB %K 8  days %K platelet production 616452 %K MK %K JPG 193 kb %X 616452 and TPO independence and synergy. BM and CB CD34+ cells were cultured in the presence of SCF and TPO. In 4 days of culture, the cells were all healthy and relatively the same size (a,b). By 8 days of culture, BM cells appeared to have a few (<1%) megakaryocytes with a size approximately four times that of a regular HSC (c). CB however, had no such cells (d). Those cells induced with 616452 for both BM and CB developed a significant number of large MKs (e,f). When CB CD34+ cells were induced with 616452 for 8 days under various cytokine conditions (g), it was found that 616452 works independently of either SCF or TPO inducing MK maturation under all conditions. (JPG 193 kb) %I figshare