%0 Figure %A Perez-Aso, Miguel %A Montesinos, M. %A Mediero, Aránzazu %A Wilder, Tuere %A Schafer, Peter %A Cronstein, Bruce %D 2015 %T Additional file 1: Figure S1. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors %U https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Apremilast_a_novel_phosphodiesterase_4_PDE4_inhibitor_regulates_inflammation_through_multiple_cAMP_downstream_effectors/4456835 %R 10.6084/m9.figshare.c.3640835_D2.v1 %2 https://springernature.figshare.com/ndownloader/files/7186088 %K TIFF 170 kb %K CGS 21680 %K intracellular cAMP levels %K novel phosphodiesterase 4 %K 2AR activation %K effectors Adenosine %K nM %K Raw 264.7 cells %K 1 μ M %K concentration %K element binding protein %K apremilast %K LPS %K 20 minutes %K 2A receptor %K PDE %K additively increase %K CGS 21680 1μ M 15 minutes %X Adenosine A2A receptor (A2AR) activation and apremilast do not additively increase responsive element binding protein (cAMP). Raw 264.7 cells were incubated with cumulative concentrations of apremilast (6 nM to 1 μM), apremilast + CGS21680 1μM 15 minutes before apremilast, or cumulative concentrations of CGS21680 alone (6 nM to 1 μM), followed by treatment with lipopolysaccharide (LPS) 1 μM for 20 minutes. Then, intracellular cAMP levels were measured as described under “Materials and methods”. Data represent means ± standard error of the mean of at least three independent experiments. (TIFF 170 kb) %I figshare