10.6084/m9.figshare.c.3639734_D4.v1
Mirko Singer
Mirko
Singer
Jennifer Marshall
Jennifer
Marshall
Kirsten Heiss
Kirsten
Heiss
Gunnar Mair
Gunnar
Mair
Dirk Grimm
Dirk
Grimm
Ann-Kristin Mueller
Ann-Kristin
Mueller
Friedrich Frischknecht
Friedrich
Frischknecht
Additional file 1: Fig. S1. of Zinc finger nuclease-based double-strand breaks attenuate malaria parasites and reveal rare microhomology-mediated end joining
Springer Nature
2015
Apicomplexa
Plasmodium
Malaria
Genetically attenuated parasites
Vaccine
Zinc-finger nucleases
Alternative end joining
Microhomology-mediated end joining
Alternative DNA repair
DSB
2015-11-17 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Fig_S1_of_Zinc_finger_nuclease-based_double-strand_breaks_attenuate_malaria_parasites_and_reveal_rare_microhomology-mediated_end_joining/4452926
Integration PCRs of parasites used in this study. a Integration of SpZFN and LsZFN. PCR with P53/P54 amplifies the whole Chr12 locus, resulting in 1448 bp in WT, 7850 bp for SpZFN and 7141 bp for LsZFN. The 5’ integration was amplified with P53/P12 or P53/P24, resulting in 1226 bp and 1610 bp for SpZFN and LsZFN, respectively. The 3’ integration was performed with P54/P55, resulting in 1181 bp. b Integration of Sp2ZFN and Ls2ZFN. PCR of the whole locus resulted in 6758 bp or 7145 bp for Sp2ZFN and Ls2ZFN, respectively. The 5’ integration was performed with P53/P56 or P53/P24, resulting in 1226 bp or 1610 bp for Sp2ZFN and Ls2ZFN. The 3’ integration was verified with P54/P10 (1982 bp). c Integration of TrapZFN and Uis4ZFN. PCR of the whole locus resulted in 6988 bp and 6970 bp for TrapZFN and Uis4ZFN, respectively. The 5’ integration was performed with P53/P22 or P53/P38, resulting in 1462 bp and 1504 bp for TrapZFN and Uis4ZFN, respectively. The 3’ integration was verified with P54/P8 (2588 bp). (PDF 1088 kb)