Additional file 3: of Chromatin-wide and transcriptome profiling integration uncovers p38α MAPK as a global regulator of skeletal muscle differentiation SegalésJessica IslamAbul KumarRoshan LiuQi-Cai Sousa-VictorPedro DilworthF. BallestarEsteban PerdigueroEusebio Muñoz-CánovesPura 2016 Supplementary Figures 1 and 2 and legends. Figure S1. Relative expression of p38α and p38β in C2C12 cells. The expression of p38α and p38β was measured by qPCR in differentiating C2C12 cells. Values are mean ± SEM of three independent set of samples. Figure S2. Nuclear p38α remains constant in proliferating and differentiating myoblasts. Expression of p38α in C2C12 cells in GM and 24 h in DM: p38α (green) and DAPI (blue). Nuclear to cytoplasm fluorescence ratio was calculated using integrated density values in ImageJ software with DAPI as nuclear boundary. Scale bar = 25 μm. (PDF 7595 kb)