10.6084/m9.figshare.c.3637409_D1.v1
Ahmed Abdelbaset-Ismail
Ahmed
Abdelbaset-Ismail
Daniel Pedziwiatr
Daniel
Pedziwiatr
Ewa Suszyńska
Ewa
Suszyńska
Sylwia Sluczanowska-Glabowska
Sylwia
Sluczanowska-Glabowska
Gabriela Schneider
Gabriela
Schneider
Sham Kakar
Sham
Kakar
Mariusz Ratajczak
Mariusz
Ratajczak
Additional file 3: Figure S3. of Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines
Springer Nature
2016
Vitamin D3
VDR
Germline tumors
Ovarian cancer
Teratocarcinoma
VSELs
2016-04-18 05:00:00
Presentation
https://springernature.figshare.com/articles/presentation/Additional_file_3_Figure_S3_of_Vitamin_D3_stimulates_embryonic_stem_cells_but_inhibits_migration_and_growth_of_ovarian_cancer_and_teratocarcinoma_cell_lines/4444601
ESD3 murine embryonic stem cells express functional VDRs. Expression of VDRs was detected in purified mRNA samples from the ESD3 cell line as well as in murine BM-derived HSCs and MNCs (Panel A) by conventional reverse transcription polymerase chain reaction (RT-PCR). Samples with water only instead of cDNA (−cDNA) and without reverse transcriptase (−RT) were used as negative controls. Representative agarose gels of the RT-PCR amplicons obtained are shown. The effect of 1,25-dihydroxyvitamin D3 on phosphorylation of p42/44 MAPK and AKTser473 intracellular pathway proteins in ESD3 cells (Panel B) was assessed. Cells (106 cells/mL) were starved for 12 h in their respective culture media containing 0.5 % BSA in an incubator and afterwards stimulated for 5 min with 1,25-dihydroxyvitamin D3 at various concentrations (0.1–100 nM) or with vehicle (DMSO) only. The experiment was carried out twice with similar results, and representative blots are shown. Panel C. Vitamin D3 does not inhibit Transwell migration of murine ESD3 cells. The experiment was performed twice with similar results. (PPT 293 kb)