%0 Figure %A Kolf, Catherine %A Song, Lin %A Helm, Jeannine %A Tuan, Rocky %D 2015 %T Additional file 4: Figure S3. of Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro %U https://springernature.figshare.com/articles/figure/Additional_file_4_Figure_S3_of_Nascent_osteoblast_matrix_inhibits_osteogenesis_of_human_mesenchymal_stem_cells_in_vitro/4427660 %R 10.6084/m9.figshare.c.3632258_D7.v1 %2 https://springernature.figshare.com/ndownloader/files/7154294 %K Mesenchymal stem cells %K Osteoblasts %K Extracellular matrix %K Osteogenesis %K Stem cell niche %X Effects of OBC co-culture on MSC osteogenic gene expression is variable. mRNA levels of osteogenic genes (OC, Runx2, ALP, and Col I) in MSC control cultures and MSC/OBC co-cultures were measured via real-time RT-PCR, and normalized to GAPDH. Each value was then expressed as a percentage of the maximum level reached for that gene in cells from a given donor within a given experiment. Values shown are the mean ± SEM from two experiments. No statistically significant differences were observed. (JPEG 238 kb) %I figshare