Additional file 4: Figure S3. of Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro Catherine Kolf Lin Song Jeannine Helm Rocky Tuan 10.6084/m9.figshare.c.3632258_D7.v1 https://springernature.figshare.com/articles/figure/Additional_file_4_Figure_S3_of_Nascent_osteoblast_matrix_inhibits_osteogenesis_of_human_mesenchymal_stem_cells_in_vitro/4427660 Effects of OBC co-culture on MSC osteogenic gene expression is variable. mRNA levels of osteogenic genes (OC, Runx2, ALP, and Col I) in MSC control cultures and MSC/OBC co-cultures were measured via real-time RT-PCR, and normalized to GAPDH. Each value was then expressed as a percentage of the maximum level reached for that gene in cells from a given donor within a given experiment. Values shown are the mean ± SEM from two experiments. No statistically significant differences were observed. (JPEG 238 kb) 2015-12-22 05:00:00 Mesenchymal stem cells Osteoblasts Extracellular matrix Osteogenesis Stem cell niche